Molecular genetic identification of arbuscular mycorrhizal fungi

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Development of new molecular markers for phylogeny and molecular identification of arbuscular mycorrhizal fungi (Glomeromycota)

Because of the high genetic variability of the nuclear-encoded ribosomal RNA genes within single spores of arbuscular mycorrhizal fungi (AMF, Glomeromycota), new alternative marker genes are needed to better resolve closely related taxa. Based on published nucleotide sequences of the mitochondrial large ribosomal subunit (mt-LSU), new fungal-specific primers were constructed and successfully us...

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DNA barcoding of arbuscular mycorrhizal fungi.

SUMMARY *Currently, no official DNA barcode region is defined for the Fungi. The COX1 gene DNA barcode is difficult to apply. The internal transcribed spacer (ITS) region has been suggested as a primary barcode candidate, but for arbuscular mycorrhizal fungi (AMF; Glomeromycota) the region is exceptionably variable and does not resolve closely related species. *DNA barcoding analyses were perfo...

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ژورنال

عنوان ژورنال: Ecological genetics

سال: 2018

ISSN: 2411-9202,1811-0932

DOI: 10.17816/ecogen16211-23